Overview:
Designed for 24 hour high throughput genetic analysis. 96 capillary electrophoresis instrument for automated sequencing of DNA, RNA and fragment analysis from 96 or 48 sample microtiter plates. Samples are automatically electrokinetically injected.

Includes:

Sequencing Analysis Software Module Kit

Genemapper® 4.0

96-well Plate Kit

ABI PRISM® 310 Genetic Analyzer Operator Training CD

Pentium IV Processor 733MHz + Dual 9.1 GB hard drives, Windows XP ® or better

Flat screen display

General Description:

Key Features

48 or 96 capillaries

24-hour unattended operation

Easy instrument set-up

Automated polymer filling and sample injection from both 96- or 48 -well microtiter plate formats

Multiple applications supported

Multiple dye detection

Capillary
The internally uncoated capillary is available in multiple lengths to provide support for multiple applications and run methodologies. The capillary arrays are specified to last for 100 runs and are designed for use with industry standard 96- and 48-well microtiter plates.

Separation Matrix
Before each run, the capillaries are automatically replenished with fresh polymer that dynamically coats the capillary wall to eliminate electro-osmotic flow.

POP-7™ Polymer

Reagents:

Applied Biosystems provides the following reagents for use on the 3730 series systems:

Sequencing Reagents

BigDye® Terminator v1.1

BigDye® Terminator v3.1

Genotyping Reagents

SNPlex Genotyping System®

SNaPshot® Multiplex Kit

Linkage Mapping Set v2.5

Genescan® Size Standards

Analysis software and algorithms:

Sample files are generated in the industry standard ABIF format

Sequencing Analysis Software for basecalling and sizecalling algorithms

GeneMapper® 4.0

Equipment Description:

Performance: ABI Basecaller Phred Q20KB Basecaller Q20 Bases (per 24 hrs)
Selected Applications Capillary Runs/Day Bases/Read Length of Read (LOR)** 3730 3730XL

TargetSeq® Resequencing 36 cm 72 400 400 1,440,000 bases 2,880,000 bases
Rapid Sequencing 36cm 40 500 550 1,056,000 bases 2,112,000 bases
Standard Sequencing 36cm 24 650 700 806,400 bases 1,612,800 bases
Extra Long Read Sequencing 50cm 8 >800 900 345,600 bases 691,200 bases bases with .l
DNA sizing 36cm 44 single base resolution 42,254 84,508
up to 500 bases with .15 SD Genotypes Genotypes

Laser:
10mW Argon-ion multi-line, single mode laser primary excitation lines: 488 and 514.5 nm.

Detection and Optics:
The ABI PRISM® 3730 Genetic Analyzer uses excitation and detection optics that provide for enhanced signal uniformity. The capillary outer diameter (od), inner diameter (id) and pitch have been optimized to minimize loss of signal due to refraction. The detection optics provide low noise, full spectrum data simultaneously from all capillaries.

Electrophoresis Unit:
Electrophoresis Voltage from 100V to 15 kV

Electrical Requirements

200 - 240V

Single Phase

>20Amps (30 Recommended)

Environmental Requirements

Operating temperature should be between 15°C to 35°C. Humidity should be between 20% - 80% (non-condensing)

Dimensions (without computer):

Width (with door closed): 60.96 cm

Width (with door open): 109.56 cm (right door open)

Depth: 55.88 cm

Height: 81 cm

Weight: approximately 120 kg