Introduction:
For high-throughput applications or busy labs, the PTC-225 or 'Tetrad' DNA Engine Tetrad may
be the best option. This allows up to eight separate blocks to run synchronous or independent
reactions. A compact footprint is maintained by housing the power supply separately outside the
chassis, good for tight spaces or robotic applications.

Features & Specifications:
· Hot Bonnet heated lid incorporates a thumbwheel to adjust height and pressure. This
feature assures tight sealing of reaction vessels even microplates during oil-free thermal
cycling

· Peltier-effect heat pumps robustly designed for repeated cycling deliver the full
temperature range of 0°-100°C electronically

· Speedy ramping rates of up to 1°C/sec in models that hold 0.5ml tubes, 96-U-well or
192-well plates; up to 1.4°/sec in models that hold 0.2ml tubes or V-well plates; up to
2.5°/sec in models with the silver-gold block.

· Temperature homogeneity: ±0.4°C within 30 seconds of arrival @ 60°C for all standard
models.

· Memory stores approximately 320 programs, and cycler comes with 14 pre-programmed
protocols. Any sequence of temperatures can be cycled up to 10,000 times; ramping
rates less than maximum may be specified.

· Extremely accurate incubations, with NIST-traceable calibration. Any temperature
including below-ambient temperatures may be held indefinitely. This feature allows
refrigeration after an unattended run.

· Parallel printer port (optionally) allows the instrument to print thermal profiles in real
time and document programs in hard copy. Additionally, an analog jack can output to a 0-
5 volt chart recorder.

· Power-failure protection will resume program after power interruptions of up to
twelve hours.

· 4-bay high-throughput thermal cycling

· Up to 8 individual sample blocks, which can be controlled independently or as a
group

· Up to 1536 samples can be processed simultaneously

· Real-time monitoring of cycling temperature

· Extraordinary temperature uniformity of ±0.4°C within 30 seconds of arrival at 90°C,
well-to-well average (±0.5°C for dual block Alphas).

· Superb thermal accuracy of blocks within ±0.3°C of NIST standard at 90°C, average
across block (±0.4°C for dual block Alphas).

· Thermal range of -5° to 105°C

· Rapid ramp rates of up to 3.0°C/sec