The RF-5301 Spectrofluorophotometer has a high-throughput optical system that employs a blazed holographic grating, photomultiplier and digital
signal processing. The RF-5301PC provides the highest level S/N ratio attainable, making it ideal for applications in such areas as
pharmaceuticals and pharmacology, food science, biochemistry and environmental monitoring.

Light Source: 150W Xenon lamp. Ozone resolving type lamp housing.
Excitation and emission monochromators: Concave, blazed holographic grating, F/2.5, 1300 grooves/mm.
Wavelength scale: 220-900nm.
Measuring wavelength range: 220-750nm and 0 order as standard. 220-900nm with the optional R928 photomultiplier.
Spectral bandwith: 6-step selection of 1.5, 3, 5, 10, 15 and 20nm. (6nm bandwith is available for half sample height on the excitation
side only.)
Wavelength accuracy: ±1.5nm.
Light source compensation: Dynode feedback system with monochromatic light monitoring.
Sensitivity: The S/N ratio is 150 or higher for the Raman line of distilled water (350nm excitation wavelength, 5nm spectral bandwidth,
and 2 second response for 98% of the full scale).
Wavelength scanning: 7-step selection of Survey (about 5500nm/min), Super (about 3000nm/min), Very Fast, Fast, Medium, Slow and Very Slow.
Wavelength slewing speed: About 20,000nm/min.
Response: 8-step selection of 0.02, 0.03, 0.1, 0.25, 0.5, 2, 4, and 8 seconds for 98% of the full scale.
Sensitivity selection: 2-steps of HIGH and LOW. (The sensitivity at HIGH is about 50 times that of LOW.)
Interface: RS-232C interface, interface for autosampler, and interface for sipper unit.
Dimensions and weight: 667W x 530D x 270H mm; 43 kg.
Power requirements: 100, 120, 220, 240V; 50/60Hz; 400VA.
Operational temperature range: 15-35°C
Operational humidity range: 40-80% (Below 70% with temperature higher than 30°C).

Software Specifications
Measurement: Excitation, emission and synchronous spectrum measurement, time-course measurement, quantitation, automatic search of optimal excitation and emission wavelengths
Data processing: Arithmetic calculation between spectra and between a spectrum and a constant, smoothing, 1st through 4th derivatives,
1/Y, logarithmic conversion, data printout (with or without activity value computation), peak pick, point pick, area calculation, averaging
(in quantitation), generation of calibration curves of 1st through 3rd order.
Filing: Save, recall, and delete data. Conversion into ASCII or CSV formats.
Data output : Automatic scale adjustment, readout of data at user-specified point, data printout (preview function provided), and selection
of colors and types of curves.
Maintenance: Macro ability for signal-to-noise ratio, monitoring of the run time of light source lamp.
User interface: ToolBox (assign icons for commonly used menu commands), Online Display, and Macro ability.
Other functions: Data exchange via clipboard, auto response control, automatic shutter.
PC requirements: IBM-PC/AT or 100% compatible; 486 or higher CPU; 16 Mbyte or larger main memory. Operates on MS-Windows version 95 or higher.